Recombinant:Article Title: Attachment culture of cortical organoids at the microwell air-liquid interface.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit Polyclonal Tubulin b 3 (TUJ1) (1:500) Abcam Cat# ab18207, RRID: AB_444319 Chicken Polyclonal Glial Fibrillary Acidic Protein (GFAP) (1:2000) Abcam Cat# ab4674, RRID: AB_304558 Goat Polyclonal Synaptophysin (Syn) (1:2000) RD Cat# AF5555, RRID: AB_2198864 Rabbit polyclonal postsynaptic density protein 95 (PSD-95) (1:1000) Thermo Fisher Cat# 51-6900, RRID: AB_2533914 Chicken polyclonal microtubule-associated protein 2 (MAP2) (1:2000) Abcam Cat# ab92434, RRID: AB_2138147 Mouse Monoclonal Vesicular Glutamate Transporter 1 (VGLUT1) (1:500) Millipore Cat# MAB5502, RRID: AB_262185 (Continued on next page) STAR Protocols 4, 102502, September 15, 2023 3 Continued REAGENT or RESOURCE SOURCE IDENTIFIER Rabbit monoclonal S100 beta (1:500) Abcam Cat# ab52642, RRID: AB_882426 Goat anti-chicken IgY H&L (Alexa Fluor 647) Abcam Cat# ab150171, RRID: AB_2921318 Donkey anti-rabbit IgG H&L (Alexa Fluor 488) Abcam Cat# ab150073, RRID: AB_2636877 Donkey anti-rabbit IgG H&L (Alexa Fluor 568) Abcam Cat# ab175470, RRID: AB_2783823 Donkey anti-goat IgG H&L (Alexa Fluor 488) Abcam Cat# ab150129, RRID: AB_2687506 Goat anti-mouse IgG H&L (Alexa Fluor 594) Abcam Cat# ab150116, RRID: AB_2650601 Donkey anti-rabbit IgG H&L (Alexa Fluor 647) Abcam Cat# ab150075, RRID: AB_2752244 Chemicals, peptides, and recombinant proteins Corning Matrigel Growth Factor Reduced (GFR) Basement Membrane Matrix, LDEV-free Corning Cat# 354230/356230 Polydimethylsiloxane (PDMS) Dow Corning Cat# SYLGARD 184 Silicone Elastomer kit Poly-L-ornithine solution Sigma Cat# P4957-50ML Neurobasal medium Thermo Fisher Cat# 21103049 BrainPhys neuronal medium STEMCELL Cat# 05790 Fluo-4, AM, cell permeant Thermo Fisher Cat# F14201 Triton X-100 Sigma Cat# X100-100ML Normal donkey serum Jackson ImmunoResearch Cat# 017-000-121 Calcein-AM Yeasen Cat# 40747-A Hoechst 33342 Yeasen Cat# 40731ES10 DAPI Sigma Cat# D9542 Agarose, ultra-low gelling temperature, molecular biology grade Sigma Cat# A2576-5G DMEM/F-12 Gibco Cat# 11330032 Penicillin/streptomycin/amphotericin B, sterile solution Leagene Cat# CA0077 Experimental models: Cell lines DXR0109B ATCC Cat# ACS-1023 H9 Wicell Cat# Wa09 Software and algorithms CALIMA F.D.W.
Membrane:Article Title: Attachment culture of cortical organoids at the microwell air-liquid interface.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit Polyclonal Tubulin b 3 (TUJ1) (1:500) Abcam Cat# ab18207, RRID: AB_444319 Chicken Polyclonal Glial Fibrillary Acidic Protein (GFAP) (1:2000) Abcam Cat# ab4674, RRID: AB_304558 Goat Polyclonal Synaptophysin (Syn) (1:2000) RD Cat# AF5555, RRID: AB_2198864 Rabbit polyclonal postsynaptic density protein 95 (PSD-95) (1:1000) Thermo Fisher Cat# 51-6900, RRID: AB_2533914 Chicken polyclonal microtubule-associated protein 2 (MAP2) (1:2000) Abcam Cat# ab92434, RRID: AB_2138147 Mouse Monoclonal Vesicular Glutamate Transporter 1 (VGLUT1) (1:500) Millipore Cat# MAB5502, RRID: AB_262185 (Continued on next page) STAR Protocols 4, 102502, September 15, 2023 3 Continued REAGENT or RESOURCE SOURCE IDENTIFIER Rabbit monoclonal S100 beta (1:500) Abcam Cat# ab52642, RRID: AB_882426 Goat anti-chicken IgY H&L (Alexa Fluor 647) Abcam Cat# ab150171, RRID: AB_2921318 Donkey anti-rabbit IgG H&L (Alexa Fluor 488) Abcam Cat# ab150073, RRID: AB_2636877 Donkey anti-rabbit IgG H&L (Alexa Fluor 568) Abcam Cat# ab175470, RRID: AB_2783823 Donkey anti-goat IgG H&L (Alexa Fluor 488) Abcam Cat# ab150129, RRID: AB_2687506 Goat anti-mouse IgG H&L (Alexa Fluor 594) Abcam Cat# ab150116, RRID: AB_2650601 Donkey anti-rabbit IgG H&L (Alexa Fluor 647) Abcam Cat# ab150075, RRID: AB_2752244 Chemicals, peptides, and recombinant proteins Corning Matrigel Growth Factor Reduced (GFR) Basement Membrane Matrix, LDEV-free Corning Cat# 354230/356230 Polydimethylsiloxane (PDMS) Dow Corning Cat# SYLGARD 184 Silicone Elastomer kit Poly-L-ornithine solution Sigma Cat# P4957-50ML Neurobasal medium Thermo Fisher Cat# 21103049 BrainPhys neuronal medium STEMCELL Cat# 05790 Fluo-4, AM, cell permeant Thermo Fisher Cat# F14201 Triton X-100 Sigma Cat# X100-100ML Normal donkey serum Jackson ImmunoResearch Cat# 017-000-121 Calcein-AM Yeasen Cat# 40747-A Hoechst 33342 Yeasen Cat# 40731ES10 DAPI Sigma Cat# D9542 Agarose, ultra-low gelling temperature, molecular biology grade Sigma Cat# A2576-5G DMEM/F-12 Gibco Cat# 11330032 Penicillin/streptomycin/amphotericin B, sterile solution Leagene Cat# CA0077 Experimental models: Cell lines DXR0109B ATCC Cat# ACS-1023 H9 Wicell Cat# Wa09 Software and algorithms CALIMA F.D.W.
Sterility:Article Title: Attachment culture of cortical organoids at the microwell air-liquid interface.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit Polyclonal Tubulin b 3 (TUJ1) (1:500) Abcam Cat# ab18207, RRID: AB_444319 Chicken Polyclonal Glial Fibrillary Acidic Protein (GFAP) (1:2000) Abcam Cat# ab4674, RRID: AB_304558 Goat Polyclonal Synaptophysin (Syn) (1:2000) RD Cat# AF5555, RRID: AB_2198864 Rabbit polyclonal postsynaptic density protein 95 (PSD-95) (1:1000) Thermo Fisher Cat# 51-6900, RRID: AB_2533914 Chicken polyclonal microtubule-associated protein 2 (MAP2) (1:2000) Abcam Cat# ab92434, RRID: AB_2138147 Mouse Monoclonal Vesicular Glutamate Transporter 1 (VGLUT1) (1:500) Millipore Cat# MAB5502, RRID: AB_262185 (Continued on next page) STAR Protocols 4, 102502, September 15, 2023 3 Continued REAGENT or RESOURCE SOURCE IDENTIFIER Rabbit monoclonal S100 beta (1:500) Abcam Cat# ab52642, RRID: AB_882426 Goat anti-chicken IgY H&L (Alexa Fluor 647) Abcam Cat# ab150171, RRID: AB_2921318 Donkey anti-rabbit IgG H&L (Alexa Fluor 488) Abcam Cat# ab150073, RRID: AB_2636877 Donkey anti-rabbit IgG H&L (Alexa Fluor 568) Abcam Cat# ab175470, RRID: AB_2783823 Donkey anti-goat IgG H&L (Alexa Fluor 488) Abcam Cat# ab150129, RRID: AB_2687506 Goat anti-mouse IgG H&L (Alexa Fluor 594) Abcam Cat# ab150116, RRID: AB_2650601 Donkey anti-rabbit IgG H&L (Alexa Fluor 647) Abcam Cat# ab150075, RRID: AB_2752244 Chemicals, peptides, and recombinant proteins Corning Matrigel Growth Factor Reduced (GFR) Basement Membrane Matrix, LDEV-free Corning Cat# 354230/356230 Polydimethylsiloxane (PDMS) Dow Corning Cat# SYLGARD 184 Silicone Elastomer kit Poly-L-ornithine solution Sigma Cat# P4957-50ML Neurobasal medium Thermo Fisher Cat# 21103049 BrainPhys neuronal medium STEMCELL Cat# 05790 Fluo-4, AM, cell permeant Thermo Fisher Cat# F14201 Triton X-100 Sigma Cat# X100-100ML Normal donkey serum Jackson ImmunoResearch Cat# 017-000-121 Calcein-AM Yeasen Cat# 40747-A Hoechst 33342 Yeasen Cat# 40731ES10 DAPI Sigma Cat# D9542 Agarose, ultra-low gelling temperature, molecular biology grade Sigma Cat# A2576-5G DMEM/F-12 Gibco Cat# 11330032 Penicillin/streptomycin/amphotericin B, sterile solution Leagene Cat# CA0077 Experimental models: Cell lines DXR0109B ATCC Cat# ACS-1023 H9 Wicell Cat# Wa09 Software and algorithms CALIMA F.D.W.
Software:Article Title: Attachment culture of cortical organoids at the microwell air-liquid interface.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit Polyclonal Tubulin b 3 (TUJ1) (1:500) Abcam Cat# ab18207, RRID: AB_444319 Chicken Polyclonal Glial Fibrillary Acidic Protein (GFAP) (1:2000) Abcam Cat# ab4674, RRID: AB_304558 Goat Polyclonal Synaptophysin (Syn) (1:2000) RD Cat# AF5555, RRID: AB_2198864 Rabbit polyclonal postsynaptic density protein 95 (PSD-95) (1:1000) Thermo Fisher Cat# 51-6900, RRID: AB_2533914 Chicken polyclonal microtubule-associated protein 2 (MAP2) (1:2000) Abcam Cat# ab92434, RRID: AB_2138147 Mouse Monoclonal Vesicular Glutamate Transporter 1 (VGLUT1) (1:500) Millipore Cat# MAB5502, RRID: AB_262185 (Continued on next page) STAR Protocols 4, 102502, September 15, 2023 3 Continued REAGENT or RESOURCE SOURCE IDENTIFIER Rabbit monoclonal S100 beta (1:500) Abcam Cat# ab52642, RRID: AB_882426 Goat anti-chicken IgY H&L (Alexa Fluor 647) Abcam Cat# ab150171, RRID: AB_2921318 Donkey anti-rabbit IgG H&L (Alexa Fluor 488) Abcam Cat# ab150073, RRID: AB_2636877 Donkey anti-rabbit IgG H&L (Alexa Fluor 568) Abcam Cat# ab175470, RRID: AB_2783823 Donkey anti-goat IgG H&L (Alexa Fluor 488) Abcam Cat# ab150129, RRID: AB_2687506 Goat anti-mouse IgG H&L (Alexa Fluor 594) Abcam Cat# ab150116, RRID: AB_2650601 Donkey anti-rabbit IgG H&L (Alexa Fluor 647) Abcam Cat# ab150075, RRID: AB_2752244 Chemicals, peptides, and recombinant proteins Corning Matrigel Growth Factor Reduced (GFR) Basement Membrane Matrix, LDEV-free Corning Cat# 354230/356230 Polydimethylsiloxane (PDMS) Dow Corning Cat# SYLGARD 184 Silicone Elastomer kit Poly-L-ornithine solution Sigma Cat# P4957-50ML Neurobasal medium Thermo Fisher Cat# 21103049 BrainPhys neuronal medium STEMCELL Cat# 05790 Fluo-4, AM, cell permeant Thermo Fisher Cat# F14201 Triton X-100 Sigma Cat# X100-100ML Normal donkey serum Jackson ImmunoResearch Cat# 017-000-121 Calcein-AM Yeasen Cat# 40747-A Hoechst 33342 Yeasen Cat# 40731ES10 DAPI Sigma Cat# D9542 Agarose, ultra-low gelling temperature, molecular biology grade Sigma Cat# A2576-5G DMEM/F-12 Gibco Cat# 11330032 Penicillin/streptomycin/amphotericin B, sterile solution Leagene Cat# CA0077 Experimental models: Cell lines DXR0109B ATCC Cat# ACS-1023 H9 Wicell Cat# Wa09 Software and algorithms CALIMA F.D.W.
|